In this study, we demonstrate that inefficient autophagy contributes to the development of atherosclerotic plaques in low-SS areas. Defective endothelial autophagy not only curbs endothelial alignment with the direction of blood flow, but also promotes an inflammatory, apoptotic, and senescent phenotype. Plaquenil cardiotoxicity Chloroquine acidic environment Rationale Sustained cardiac hypertrophy often leads to heart failure HF. Understanding the regulation of cardiomyocyte growth is crucial for the treatment of adverse ventricular remodeling and HF. Cell division cycle 20 CDC20 is an anaphase-promoting. Mice were treated with chloroquine 50 mg/kg for 5 days to block lysosomal function. Densitometry analysis showed increased LC3-II relative to GAPDH in CryAB R120G. Immunofluorescence analysis of heart sections showing punctate LC3 staining in 4-month-old mice. DAPI blue was used to identify nuclei. Original magnification, ×60. A 'read' is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the full-text. Altogether, these findings underline the role of endothelial autophagic flux activation by SS as an atheroprotective mechanism. Furthermore, genetic inactivation of endothelial autophagy in a murine model of atherosclerosis increases plaque burden exclusively in high-SS areas that are normally resistant to atherosclerotic plaque development. Lc3 aging gapdh chloroquine heart Lysosomal dysfunction and impaired autophagy underlie the., JCI - Enhanced autophagy ameliorates cardiac proteinopathy Chloroquine mode of actionHow long should i take plaquenilPlaquenil and the flu Aging is associated with hypermethylation of autophagy genes in macrophages Article PDF Available in Epigenetics 11500-00 February 2016 with 244 Reads How we measure 'reads' Aging is associated with hypermethylation of autophagy.. PDF CDC20 regulates cardiac hypertrophy via targeting LC3.. Role of autophagy in modulating post-maturation aging of.. Nov 15, 2018 Rationale Sustained cardiac hypertrophy often leads to heart failure HF. Understanding the regulation of cardiomyocyte growth is crucial for the treatment of adverse ventricular remodeling and HF. Cell division cycle 20 CDC20 is an anaphase-promoting. C Western blots of LC3-PE formation in HEK293 cells transfected with GAPDH siRNA and starved of glucose or treated with AICAR in the presence of chloroquine CQ. D Intracellular p62 levels in glucose-starved HEK293 cells transfected with GFP-GAPDH or GFP-NES-GAPDH after 48 hr culture with GAPDH siRNA. Autophagy is commonly measured by western blotting for LC-II, in which it is generally accepted that an increase in LC3-II is associated with an increase in autophagy although increased LC3-II could also result from impaired autophagic flux. Studies of autophagy in the aging heart have yielded inconsistent results.